Journal: Journal of cell science
Article Title: Rab2a and Rab27a cooperatively regulate the transition from granule maturation to exocytosis through the dual effector Noc2.
doi: 10.1242/jcs.195479
Figure Lengend Snippet: Figure 6. Effects of overexpression of Noc2 and its mutants on insulin secretion
Article Snippet: The following commercially purchased antibodies were also used: rabbit polyclonal antibodies toward FLAG (F7425, Sigma-Aldrich), hemagglutinin (HA; 561, MBL), green fluorescent protein (GFP; 598, MBL), Rab27a/b (18975, IBL), Noc2 (15297-1-AP, Proteintech), Rab2a (15420-1-AP, Proteintech), ICA69 (ab81500, Abcam), and PICK1 (ab3420, Abcam); and mouse monoclonal antibodies toward Rab3 (610379, BD Biosciences), EEA1 (610457, BD Biosciences), TGN38 (610849, BD Biosciences), PDI (MA3-018, Affinity BioReagents), α-tubulin (T5168, Sigma-Aldrich), and proinsulin (clone 3A1; ab8301, Abcam).
Techniques: Over Expression